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应用ＡＢＣ免疫组化法，检测６８例大肠癌Ｐ５３蛋白和增值细胞核抗原的表达。结果显示，大肠癌组织中Ｐ５３蛋白阳性表达率为６４７％（４４／６８），３０例正常组织无Ｐ５３阳性表达。Ｐ５３阳性表达的大肠癌组织增殖细胞核抗原计数值较阴性者明显增高（Ｐ＜００１）。进一步观察发现，大肠癌Ｄｕｋｅ’ｓ，分期和五年随访复发率与Ｐ５３蛋白阳性表达及增殖细胞核抗原计数值呈正相关。结果提示，联合检测Ｐ５３增殖细胞核抗原表达对大肠癌诊断、分期及预后判断有重要的参考价值。
ABC immunohistochemical method was used to detect the expression of P53 protein and the proliferation of nuclear antigen in 68 cases of colorectal cancer. The results showed that the positive expression rate of P53 protein was 64.7% (44/68) in colorectal cancer tissues, and there was no positive expression of P53 in 30 normal tissues. P53-positive colorectal cancer tissues had significantly higher proliferating cell nuclear antigen counts than those negative (P<001). Further observations revealed that the recurrence rate of Duke’s in colorectal cancer was positively correlated with the positive expression of P53 protein and the count of proliferating cell nuclear antigen. The results suggest that the combined detection of P53 proliferating cell nuclear antigen expression has important reference value for the diagnosis, staging and prognosis of colorectal cancer.