目的探讨let-7a通过靶向调控周期素D2(cyclin D2)对胶质瘤细胞增殖的影响。方法在U87和U251胶质瘤细胞中转染入化学合成的let-7a模拟物上调let-7a表达,荧光素酶报告基因实验检测let-7a与周期素D2的靶向调控关系,Western blot检测转染let-7a后周期素D2的表达情况,MTT和流式细胞术检测let-7a对胶质瘤细胞增殖的抑制作用。结果在胶质瘤细胞中周期素D2是let-7a的靶基因,过表达的let-7a可下调周期素D2表达和抑制胶质瘤细胞增殖。结论let-7a可通过靶向调控周期素D2而抑制胶质瘤细胞增殖。 Objective To investigate the effect of let-7a on the proliferation of glioma cells by targeting cyclin D2. METHODS: The let-7a expression was induced by up-regulation of let-7a in U87 and U251 glioma cells. Luciferase reporter assay was used to detect the let-7a expression of let-7a and cyclin D2. Western blot analysis The expression of cyclin D2 after let-7a transfection was detected by MTT assay and flow cytometry. The inhibitory effect of let-7a on glioma cell proliferation was detected. Results Cyclin D2 was the target gene of let-7a in glioma cells. Overexpression of let-7a down-regulated the expression of cyclin D2 and inhibited the proliferation of glioma cells. Conclusion let-7a can inhibit the proliferation of glioma cells by targeting cyclin D2.