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目的探讨miR-491在肝癌组织中的表达、临床意义及可能的分子机制。方法实时定量PCR检测miR-491在肝癌及癌旁组织中的表达,MTT法检测MHCC-97H细胞的增殖,TranswellTM实验检测MHCC-97H细胞的侵袭及迁移,Western blot法及免疫组织化学染色检测miR-491下游潜在靶点Xklp2靶蛋白(TPX2)的表达。结果 miR-491在肝癌组织中表达水平显著低于相应癌旁组织;肝癌组织中miR-491低表达与肿瘤大小、TNM分期、门静脉侵犯及Edmondson分级显著相关;低表达miR-491患者3年生存率明显低于高表达组;miR-491可显著抑制MHCC-97H细胞的增殖、侵袭及迁移,并下调TPX2的蛋白水平;miR-491低表达组TPX2蛋白水平明显高于miR-491高表达组,相关性分析显示肝癌组织中miR-491与TPX2蛋白表达呈显著负相关。结论 miR-491在肝癌组织中表达下调并与肝癌恶性临床病理特征有关,miR-491抑制肝癌细胞增殖、侵袭及迁移的作用可能与下调TPX2表达有关。
Objective To investigate the expression, clinical significance and possible molecular mechanisms of miR-491 in hepatocellular carcinoma. Methods The expression of miR-491 in hepatocellular carcinoma and its adjacent tissues was detected by real-time PCR. The proliferation of MHCC-97H cells was detected by MTT assay. The invasion and migration of MHCC-97H cells were detected by TranswellTM assay. The expression of miR-491 was detected by Western blot and immunohistochemical staining -491 potential target downstream Xklp2 target protein (TPX2) expression. Results The expression of miR-491 in HCC tissues was significantly lower than that in corresponding paracancerous tissues. The low expression of miR-491 in HCC tissues was significantly associated with tumor size, TNM stage, portal vein invasion and Edmondson classification. The 3-year survival rate of miR-491 patients with low expression of miR- MiR-491 significantly inhibited the proliferation, invasion and migration of MHCC-97H cells and down-regulated the protein level of TPX2. The level of TPX2 protein in miR-491 low expression group was significantly higher than that in miR-491 high expression group The correlation analysis showed that there was a significant negative correlation between the expression of TPX2 and miR-491 in HCC tissues. Conclusions miR-491 is down-regulated in HCC tissues and is associated with clinicopathological features of HCC. The role of miR-491 in inhibiting the proliferation, invasion and migration of HCC cells may be related to the down-regulation of TPX2 expression.