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培养了由２６个小麦（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍ，２ｎ＝６ｘ＝４２）品种（系）用鸭茅状摩擦禾（Ｔｒｉｐｓａｃｕｍｄａｃｔｙｌｏｉｄｅｓ，２ｎ＝４ｘ＝７２）的花粉授粉１４天后获得的６４５个未成熟的胚。结果表明，未成熟胚培养的植株再生频率与胚来源的小麦基因型的杂交亲和性无关。胚的发育程度直接影响胚培养的结果。离体时分化完全的未成熟胚在无激素的培养基上可以迅速萌发成苗，而分化未完全的小胚在无激素的培养基上分化进程不能继续，而且在无激素补充的情况下，萌发过程一旦起动，即使将这些胚转至补加了激素的胚分化培养基上，分化过程也不能再补救。讨论了提高未成熟胚培养成苗率的关键和措施。
645 immature embryos obtained after 14 days of pollination of 26 wheat (Triticum aestivum, 2n = 6x = 42) varieties (lines) with pollination of Tripsacum dactyloides (2n = 4x = 72) were cultured. The results show that the frequency of plant regeneration in immature embryos is not related to the hybridization affinity of embryo-derived wheat genotypes. The degree of embryo development directly affects the results of embryo culture. In vitro differentiation of immature embryos in hormone-free medium can rapidly germinate, and incomplete differentiation of small embryos in the hormone-free medium differentiation process can not continue, and in the absence of hormone replacement, Once the germination process is started, the differentiation process can not be remedied even if these embryos are transferred to embryogenic differentiation medium supplemented with hormones. The key and measures to improve the seedling rate of immature embryo culture are discussed.