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目的建立稳定可靠的颞叶癫痫动物模型,摸索并证实建模规律。方法选用56只健康SD大鼠,雌雄近半,随机分配为试验组48只,对照组8只氯化锂3 mmol/kg腹腔注射预处理;18～20h后首剂注射匹罗卡品30mg/kg,此后篛隔30分钟注射10mg/kg追加剂量,极量为60 mg/kg,直至出现癫痫持续状态(SE);SE 1 h后地西泮10 mg/kg止痉,2 h后注射生理盐水5 mL/只,2/d,连续2d;SE后次日起灌胃鸡蛋牛奶糊7 mL/只,2/d,连续6 d:观察大鼠行为学改变。对照组大鼠除用等量生理盐水代替匹罗卡品外,其余处理措施均相同。结果大鼠成功点燃44只,病死10只,造模总成功只数为34。雌雄大鼠点燃只数相近,雄鼠病死只数低于雌鼠。结论中等剂量首剂,不超过3次小剂量累加腹腔注射匹罗卡品造模简易有效,可操作性强,效应匀齐。改良护理方法可明显降低病死率,雄鼠较雌鼠更适于造模。
Objective To establish a stable and reliable animal model of temporal lobe epilepsy, to explore and confirm the modeling rules. Methods Fifty-six healthy SD rats were selected and randomly divided into experimental group (n = 48) and control group (n = 8). Lithium chloride 3 mmol / kg was injected intraperitoneally for pretreatment. Pilocarpine 30 mg / kg , Followed by an interval of 30 minutes after injection of 10mg / kg additional dose, the maximum amount of 60 mg / kg, until the status epilepticus (SE); SE 1 h after diazepam 10 mg / kg Zhijing, 2 h after injection of saline 5 mL / only, 2 / d, continuous 2d; SE from the next day after the intragastric administration of egg milk paste 7 mL / only, 2 / d, 6 consecutive days: observed behavioral changes. The rats in control group except the same amount of saline instead of pilocarpine, the rest of the treatment measures are the same. Results In the experiment, 44 rats were successfully ignited and 10 died. The total success rate of modeling was only 34. Male and female rats lit only a similar number of male rats died less than the number of female rats. Conclusion The first dose of medium dose, no more than three small doses of intraperitoneal injection of pilocarone accumulated simple and effective modeling, maneuverability, uniform effect. Improved nursing methods can significantly reduce the fatality rate, male rats more suitable for female models than modeling.