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目的:观察顺铂、5-氛尿嘧啶、长春新碱配伍在体外对人肝癌细胞株(7721)的相互作用.方法:采用MTT法利用中效原理判定两药合用的效果.结果:3种药物单独应用时随着药物剂量增加,其效应也增加;两药大剂量(大于中效剂量)合用时可产生拮抗作用(CI>1);小剂量(小于中效剂量)合用时可产生协同作用(CI>1);两药合用时的浓度比例及给药时间次序不同均会影响效应大小.结论:上述3种药物任何两种合用小剂量产生协同作用,大剂量产生拮抗作用,且效应大小与两药浓度比例及给药时间次序有关.
OBJECTIVE: To observe the interaction between cisplatin, 5-uracil, and vincristine in vitro on human hepatoma cell line (7721). Methods: The MTT method was used to determine the effect of the two drugs in combination with the principle of intermediate effect. The effects of the drug when used alone are increased with the increase of the drug dose; the antagonistic effect (CI>1) can be produced when large doses of the two drugs (greater than the intermediate dose) are used together; and the synergy can be produced when the low dose (less than the intermediate dose) is used together. The effect (CI>1); the concentration ratio of the two drugs combined and the order of the administration time will influence the effect size. Conclusion: Any two of the above three drugs combined with a small dose produce a synergistic effect, and a large dose produces an antagonistic effect. The size is related to the ratio of the two drug concentrations and the time sequence of administration.