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目的探讨脱嘌呤/脱嘧啶核酸内切酶(APEl)和腺苷酸二磷酸核糖基转移酶(APDRT)的基因多态与慢性苯中毒易感性的关系。方法采用病例对照研究,以152名苯中毒工人为病例组,152名接触苯而无中毒表现的工人为对照组。应用创造酶切位点的限制性片断长度多态检测技术(CRS-RFLP)检测APE1基因Asp148Glu位点和ADPRT基因Val762Ala位点的多态性。结果APE1 Asp148Glu多态与ADPRT Val762Ala多态的各基因型在病例组和对照组的分布差异无统计学意义(P＞0．05)；携带APE1 Asp148Glu Asp/Asp基因型的饮酒个体比携带该基因型的不饮酒个体发生慢性苯中毒的危险性升高(OR=4．13,95%CI：1．07～15．85,P=0．03)；多因素Logistic回归分析提示,吸烟对慢性苯中毒发病风险具有一定的修饰作用(OR=0．33,95%CI：0．14～0．75,P=0．01)。结论APE1 Asp148Glu和ADPRT Val762Ala位点多态与慢性苯中毒的发病风险没有关系。饮酒与APE1 Asp148Glu多态可能存在联合作用,仍需进一步研究确定。
Objective To investigate the relationship between polymorphisms of apurinic / apyrimidinic endonucleases (APE1) and adenosine diphosphate ribosyltransferase (APDRT) and susceptibility to chronic benzene poisoning. Methods A case-control study was conducted in which 152 workers with benzene poisoning were selected as the case group and 152 workers exposed to benzene without toxicity were used as the control group. The polymorphisms of Asp148Glu site of APE1 gene and Val762Ala site of ADPRT gene were detected by restriction fragment length polymorphism (CRS-RFLP). Results There was no significant difference in the distribution of APE1 Asp148Glu polymorphism and ADPRT Val762Ala polymorphism between the case group and the control group (P> 0.05). Compared with the case of drinking alcohol carrying APE1 Asp148Glu Asp / Asp genotype (OR = 4.13, 95% CI: 1.07 ~ 15.85, P = 0.03). The multivariate Logistic regression analysis showed that smoking had a significant effect on the chronic The risk of benzene poisoning has some modification (OR = 0.33, 95% CI: 0.14 ~ 0.75, P = 0.01). Conclusion The polymorphisms of APE1 Asp148Glu and ADPRT Val762Ala are not related to the risk of chronic benzene poisoning. Alcohol and APE1 Asp148Glu polymorphism may exist in combination, still need further study to determine.