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目的分析黄连素对体外变异链球菌生长、产酸、粘附的影响,探讨其防龋作用。方法采用微量肉汤稀释法进行最小抑菌浓度(MIC)的测定;然后通过试管粘附法测定不同浓度药液对变异链球菌粘附作用的影响;最后计算不同浓度药液作用24h后pH值的变化。结果黄连素对变异链球菌的MIC为1.25mg/mL,MBC为5.00mg/mL。实验组对变异链球菌的粘附及产酸的抑制作用随着药物浓度的增加而增强,与阴性对照组相比差异有统计学意义(P<0.05)。结论黄连素可抑制体外变异链球菌的生长、产酸及粘附。
Objective To analyze the effects of berberine on the growth, acid production and adhesion of Streptococcus mutans in vitro and its anticaries effect. Methods The minimal inhibitory concentration (MIC) was determined by the broth microdilution method. The effect of different concentrations of drug solution on the adhesion of Streptococcus mutans was determined by tube adhesion method. Finally, the pH value The change. Results berberine Streptococcus mutans MIC was 1.25mg / mL, MBC was 5.00mg / mL. Experimental group on Streptococcus mutans adhesion and acid production inhibition with the drug concentration increased, compared with the negative control group, the difference was statistically significant (P <0.05). Conclusion Berberine inhibits the growth, acid production and adhesion of Streptococcus mutans in vitro.