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利用同步荧光光谱技术,研究了pH为10.0时,Cu2+离子与明胶的相互作用,推导出含有n个键合位置的明胶大分子与Cu2+离子的结合公式;用明胶中两种荧光基团的荧光强度变化数据,线性拟合出Cu2+离子与明胶的结合常数K和结合位点数n.讨论了Cu2+离子对明胶分子构象的影响.非线性拟合结果与实验值相当吻合.
Synchronous fluorescence spectroscopy was used to study the interaction between Cu2 + ion and gelatin at pH 10.0. The binding formula between gelatin macromolecule containing n binding sites and Cu2 + ion was deduced. The fluorescence spectra of two fluorophores Intensity change data, the binding constants K of Cu2 + ions and the number of binding sites n were fitted by linearity.The effects of Cu2 + ions on the molecular conformation of gelatin were discussed.The nonlinear fitting results were in good agreement with the experimental data.