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为了考察Cu2+的存在能否影响槲皮素对牛血清白蛋白(BSA)的荧光猝灭机理、槲皮素与BSA的结合常数及槲皮素与BSA之间的作用力类型,运用荧光光谱法测试了Cu2+与BSA作用、槲皮素与BSA的相互作用及Cu2+存在时槲皮素与BSA的相互作用过程中BSA的荧光发射光谱,以Stern-Volmer方程分析BSA的荧光猝灭机理,以双对数方程lg[(F0-F)/F]=Iga+nlg[Q]计算结合常数KA,以Ross理论确定作用力类型.结果表明尽管Cu2+与BSA之间有一定的相互作用,但Cu2+与槲皮素及BSA共存时,Cu2+不改变槲皮素对BSA的荧光猝机理、不改变槲皮素与BSA结合作用的作用力类型.但Cu2+的加入,使槲皮素与BSA之间的结合常数KA减小.当Cu2+存在时,消弱了槲皮素和BSA的结合程度.“,”In order to investigate the presence of Cu2+ whether has an effect on the fluorescence quenching mechanism of quercetin on bovine serum albumin (BSA),the binding constant(KA) between quercetin and BSA,and the type of acting force between quercetin and BSA,the fluorescence spectroscopy method were applied to test the fluorescence emission spectra of BSA under different conditions respectively in the presence of Cu2+,the presence of quercetin and the coexistence of Cu2+ and quercetin.The fluorescence quenching mechanism was analyzed by Stern-Volmer equation.The binding constant KA was Calculated by the double logarithmic equations,namely lg [(F0-F)/F]=lgKa+r/g[Q].The force types were determined by Ross theory.The results showed that although there was some interaction between Cu2+ and BSA,Cu2+ neither affected the mechanism of fluorescence quenching mechanism of quercetin on BSA,nor changed the type of force between quercetin and BSA.However when the Cu2+ was present,the binding constant between quercetin and BSA would decrease.It was concluded that the presence of Cu2+had weakened the binding degree of quercetin and BSA.