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目的:探讨芦荟总苷(Total Glycoside of Aloe)的提取条件以及芦荟总苷对四氯化碳致小鼠急性肝损伤的保护作用。方法:根据设计的工艺流程,通过对浸泡时间和溶剂用量的考察,探讨芦荟总苷的最佳提取条件。以腹腔注射四氯化碳(CCl4)致小鼠急性肝损伤为模型,观察芦荟总苷提取物对小鼠血清丙氨酸氨基转移酶(ALT)及天门冬氨酸氨基转移酶(AST)活性的影响。结果:库拉索芦荟用60%的乙醇浸泡24小时,溶剂用量为4倍生药时提取得到的芦荟总苷量最多。芦荟总苷能显著降低CCl4致急性肝损伤小鼠血清AST和ALT的活性。结论:芦荟总苷对CCl致小鼠急性肝损伤具有一定的保护作用。
Objective: To investigate the extraction conditions of Total Glycoside of Aloe and the protective effect of aloe glycoside on acute hepatic injury induced by carbon tetrachloride in mice. Methods: According to the design process, the optimum extraction conditions of Aloe total glycosides were investigated by examining the soaking time and solvent dosage. Acute hepatic injury in mice induced by intraperitoneal injection of carbon tetrachloride (CCl4) was used as a model to observe the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice. Impact. RESULTS: Aloe vera was soaked in 60% ethanol for 24 hours, and the amount of total aloe glycosides was the highest when the solvent amount was 4 times crude drug. Aloe total glycosides can significantly reduce serum AST and ALT activity in mice with acute hepatic injury induced by CCl4. Conclusion: Aloe total glycoside has protective effect on acute liver injury induced by CCl in mice.