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目的探讨次声作用后鼠脑CA1区mGluR1α和mGluR5表达改变的规律，并观察其拮抗剂MCPG的作用。方法160只SD大鼠随机分为次声作用组及MCPG治疗组。两组再分为对照组及次声作用1次、7次和14次组。用8Hz、130dB的次声按规定次数，每次作用2 h。用免疫组织化学染色和原位杂交的方法检测mGluR1α和mGluR5的表达，光镜下观察MCPG治疗后神经元形态的改变。结果与对照组相比较，次声作用1次后，mGluR1α与mGluR5的阳性细胞数和吸光(A)值即改变( P∨0.05)；7次组改变最显著( P∨0.01)；14次组恢复至正常水平。形态学研究证实，MCPG对CA1区神经元有明显的保护作用。结论次声作用可通过mGluR1α和mGluR5介导兴奋性神经毒作用, 其活性的改变是导致次声性脑损害的因素之一，MCPG对次声性脑损伤具有保护作用。
Objective To investigate the changes of mGluR1α and mGluR5 expression in brain CA1 area after infrasound, and to observe the effect of its antagonist MCPG. Methods 160 SD rats were randomly divided into infrasound group and MCPG treatment group. The two groups were further divided into control group and infrasound 1, 7 and 14 groups. With 8Hz, 130dB infrasound by the required number of times, each role 2 h. The expression of mGluR1α and mGluR5 were detected by immunohistochemical staining and in situ hybridization. The morphological changes of neurons were observed under light microscope. Results Compared with the control group, the number of positive cells and absorbance (A) of mGluR1α and mGluR5 changed after infrasound for one time (P ∨ 0.05), and the changes were most significant in the seventh group (P ∨ 0.01) Return to normal level Morphological studies confirmed that MCPG has a significant protective effect on neurons in CA1 area. Conclusions Infrasound can mediate excitatory neurotoxicity via mGluR1α and mGluR5. The change of its activity is one of the factors leading to infrasound of brain damage. MCPG has a protective effect on infrasonic injury.