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目的建立黄连药材HPLC指纹图谱分析方法及含量测定方法,比较分析不同栽培条件、不同产地和不同炮制方法制备的黄连药材的质量。方法采用HPLC-UV方法建立了黄连药材指纹图谱,应用HPLC-MS联用技术指认了主要色谱峰;并对黄连药材中的小檗碱、巴马汀和药根碱进行含量测定。结果指认了黄连药材指纹图谱中7个主要的色谱峰,含量测定结果表明不同的栽培条件中,遮荫条件、生长年限对黄连中小檗碱、巴马汀和药根碱的含量影响较大,而种植密度的影响不是很明显;现代生态技术栽培的黄连中这3种生物碱的含量均高于对照药材或与之基本相当。不同产地的黄连中这3种生物碱的含量差别较大。应用不同炮制方法制备的黄连中这3种生物碱的含量差别不明显。结论现代生态栽培技术替代传统人工搭棚方式栽培黄连是可行的,同时为科学炮制黄连提供依据。
Objective To establish a method for the HPLC fingerprinting and determination of Rhizoma Coptidis Rhizoma Coptidis, and to compare and analyze the quality of Rhizoma Coptidis rhizome prepared by different cultivation conditions, different origins and different processing methods. Methods The fingerprints of Rhizoma Coptidis were established by HPLC-UV method. The main chromatographic peaks were identified by HPLC-MS. The content of berberine, palmatine and jatrorrhizine in Rhizoma Coptidis were determined. The results identified 7 major chromatographic peaks in the chromatograms of Rhizoma Coptidis. The results showed that shading conditions and growth years had a significant effect on the content of berberine, palmatine and jatrorrhizine in Coptis chinensis. The effect of planting density is not very obvious; the contents of these three alkaloids in Coptis chinensis cultivated in modern ecological techniques are all higher than or comparable to the control herbs. The content of these three alkaloids in the Coptis chinensis from different origins varies greatly. The contents of these three alkaloids in Coptis chinensis prepared by different processing methods were not significantly different. Conclusion It is feasible to cultivate Coptis chinensis using modern ecological cultivation techniques instead of traditional artificial scaffolding, and provide scientific basis for the scientific processing of Coptis chinensis.