论文部分内容阅读
目的研究系统性红斑狼疮(SLE)伴贫血患儿体内的血清肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)水平。方法对SLE患儿和正常儿童体内的TNF-α和IFN-γ水平进行检测,并观察TNF-α和IFN-γ水平对红系造血祖细胞CFU-E与BFU-E集落形成的影响。结果正常儿童体内的TNF-α和IFN-γ水平明显低于SLE伴贫血患儿。TNF-α和IFN-γ会对正常骨髓CFU-E与BFU-E集落的形成产生较为明显的抑制作用,且抑制作用会随着浓度的增加而增强。结论 SLE患儿血清中存在着能对造血干细胞产生抑制的物质;TNF-α和IFN-γ可能是引发SLE患儿贫血的因素。
Objective To investigate the levels of serum tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in children with systemic lupus erythematosus (SLE) and anemia. Methods The levels of TNF-α and IFN-γ in children with SLE and normal children were detected. The effects of TNF-α and IFN-γ on the formation of CFU-E and BFU-E colonies of erythroid hematopoietic progenitor cells were observed. Results The levels of TNF-α and IFN-γ in normal children were significantly lower than those in children with SLE and anemia. TNF-|Áand IFN-|Ãhave markedly inhibited the formation of CFU-E and BFU-E colonies in normal bone marrow, and the inhibitory effect will be enhanced with the increase of concentration. Conclusion SLE children have the ability to inhibit the hematopoietic stem cells in the serum. TNF-α and IFN-γ may play an important role in the pathogenesis of anemia in children with SLE.