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目的探讨抗核抗体(ANA)、抗可提取核抗原抗体(ENA)及抗双链DNA抗体(dsDNA)检测在系统性红斑狼疮(SLE)中的阳性率极其联检的意义。方法 ANA和dsDNA采用间接免疫荧光法(IIF法),ENA采用欧蒙印记法分别对89例SLE患者进行检测。结果 SLE各种抗体阳性率不同:ANA、dsDNA和ENA抗体在SLE阳性率分别为81%、51%和70%,ENA中抗核小体抗体(AnuA)、Sm抗体、抗核糖体P蛋白抗体(rRNP)、u1RNP抗体、SSA抗体、SSB抗体、Scl-70抗体、Jo-1抗体、PCNA抗体、抗组蛋白抗体(Hist)抗体阳性率分别为63%、27%、13%、31%、45%、16%、1%、0%、2%和31%;同时SLE活动期患者与非活动期患者AnuA、dsDNA阳性率差异有统计学意义。结论各项自身抗体在SLE中的阳性率不同,所揭示的临床意义也不同;dsDNA抗体和AnuA抗体与疾病活动性密切相关;自身抗体联合检测大大提高了SLE诊断的敏感性和特异性。
Objective To explore the significance of the positive detection rate of anti-nuclear antibody (ANA), anti-extractable nuclear antigen antibody (ENA) and anti-double-stranded DNA antibody (dsDNA) in systemic lupus erythematosus (SLE) Methods ANA and dsDNA were detected by indirect immunofluorescence method (IIF method), and ENA’s method was used to detect 89 SLE patients respectively. Results The positive rates of SLE antibodies were different: the positive rates of SNA, ANA, dsDNA and ENA were 81%, 51% and 70% respectively in SLE, AnuA, Sm and anti-ribosomal protein P in ENA The positive rates of rRNP, u1RNP, SSA, SSB, Scl-70, Jo-1, PCNA and Hist were 63%, 27%, 13% and 31% 45%, 16%, 1%, 0%, 2% and 31% respectively. The positive rates of AnuA and dsDNA in SLE patients and inactive patients were also significantly different. Conclusions The positive rates of all the autoantibodies in SLE are different, and the clinical significance is also different. The dsDNA and AnuA antibodies are closely related to disease activity. The combination of autoantibodies can greatly improve the sensitivity and specificity of SLE diagnosis.