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为探讨巨噬细胞集落刺激因子抗单核巨噬细胞过氧化损伤的机理 ,以富含巨噬细胞集落刺激因子的L92 9细胞条件培养基作为细胞因子来源 ,采用酶活性测定、反转录聚合酶链反应等技术 ,研究了巨噬细胞集落刺激因子对小鼠巨噬细胞系RAW2 6 4.7细胞谷胱甘肽过氧化物酶活性及mRNA表达的影响。结果发现 ,巨噬细胞集落刺激因子可以提高RAW2 6 4.7细胞硒谷胱甘肽过氧化物酶及非硒谷胱甘肽过氧化物酶活性 ,并增强RAW2 6 4.7细胞血浆谷胱甘肽过氧化物酶mRNA的表达 ,而对RAW2 6 4.7细胞磷脂氢过氧化物谷胱甘肽过氧化物酶的表达无诱导作用
To investigate the mechanism of macrophage colony-stimulating factor against peroxisome proliferator-activated monocyte-macrophage injury, L92 9 cell culture medium enriched in macrophage colony-stimulating factor was used as the source of cytokines. The enzyme activity determination, reverse transcription polymerization The effects of macrophage colony-stimulating factor on glutathione peroxidase activity and mRNA expression in murine macrophage cell line RAW264.7 were studied. The results showed that macrophage colony-stimulating factor can increase the activity of selenium glutathione peroxidase and non-selenium glutathione peroxidase in RAW264.7 cells and increase the glutathione peroxidation in RAW264.7 cells But not to the expression of phospholipid hydroperoxide glutathione peroxidase in RAW264.7 cells