论文部分内容阅读
目的观察以“浊毒”立论的肝复健方对猪血清诱导的大鼠肝纤维化(HF)肝脏转化生长因子-β1(TGF-β1)及其Ⅱ型受体(TβRⅡ)mRNA水平和Smad2/3、Smad4、Smad7蛋白表达的影响。方法SD大鼠腹腔注射猪血清0.5 mL,每周2次,连续8周制备HF模型,每天灌胃分别给予不同剂量的肝复健方(15.0、22.5、30、0 g/kg)和秋水仙碱(0.154 g/kg)治疗至12周末。实验结束后,RT-PCR法检测肝组织TGF-β1及其受体TβRⅡmRNA的表达,Weston-blot法检测肝组织Smad2/3、Smad4、Smad7蛋白的表达。结果与正常对照组相比,模型组TGF-β1、TβRⅡ及Smad2/3、Smad4表达明显增强,Smad7表达明显下降;经肝复健方治疗后大鼠肝脏TGF-β1及其受体TβRⅡ表达均比模型组减弱,Smad2/3、Smad4的表达减弱而Smad7蛋白的表达增强。结论肝复健方能抑制TGF-β1及其受体TβRⅡmRNA的表达,下调Smad2/3、Smad4蛋白表达,上调Smad7蛋白的表达。肝复健方对大鼠HF肝脏TGF-β/Smad信号通路有明显的影响,这可能是其治疗HF的机制之一。
Objective To observe the effect of Ganfubian Recipe (DMF) on the serum level of hepatic TGF-β1 and its receptor type Ⅱ receptor (TβRⅡ) mRNA induced by porcine serum in rat liver fibrosis (HF) And Smad2 / 3, Smad4, Smad7 protein expression. Methods SD rats were injected intraperitoneally with 0.5 mL of porcine serum twice a week for 8 weeks. HF model was established by intraperitoneal injection of different doses of Ganfukang (15.0,22.5,30,0 g / kg) Base (0.154 g / kg) treatment until the 12th weekend. After the experiment, the expression of TGF-β1 and its receptor TβRⅡmRNA in liver tissue was detected by RT-PCR. The expression of Smad2 / 3, Smad4 and Smad7 protein in liver tissue was detected by Weston-blot. Results Compared with the normal control group, the expression of TGF-β1, TβRⅡ, Smad2 / 3 and Smad4 in the model group was significantly increased and the expression of Smad7 was significantly decreased. The expression of TGF-β1 and its receptor TβRⅡ Compared with the model group, Smad2 / 3, Smad4 expression decreased and Smad7 protein expression increased. Conclusion Ganfukang can inhibit the expression of TGF-β1 and its receptor TβRⅡmRNA, down-regulate the expressions of Smad2 / 3 and Smad4, and up-regulate the expression of Smad7. Liver Recipe has a significant effect on the TGF-β / Smad signaling pathway in rat HF liver, which may be one of the mechanisms of its treatment of HF.