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本文建立了HPLC 法测定兔血浆中甲基莲心碱血药浓度的方法,甲基莲心碱的血药浓度在0.0625～0.25μg/ml 范围内,线性关系良好(r=0.9991)方法回收率日内,日间均大于99%,变异系数小于4%,用本法测定了兔单剂量(10mg/kg)静脉给药后血药浓度经时变化,结果符合三室开教模型,其动力学参数为:π=0.736min~(-1),α=0.0457min~(-1);β=0.0065min~(-1);Vc=0.6163L/kg;T_(1/2)π=0.942min;T_(1/2)α=15.167min;T_(1/2)B=107.069min,AUC=89.352(μg·min)/ml;CL=0.112L/(kg·min)。
This article established a HPLC method for the determination of plasma neferine plasma concentration of neferine, neferine plasma concentration in the range of 0.0625 ~ 0.25μg / ml, a good linear relationship (r = 0.9991) recovery rate of the method, Daytime were more than 99%, the coefficient of variation of less than 4%, using this method to determine the rabbit single dose (10mg / kg) after intravenous administration of plasma concentration over time, the results in line with the three-chamber prophylaxis model, the kinetic parameters: π = 0.736min -1, α = 0.0457min -1; β = 0.0065min -1; Vc = 0.6163L / kg; T 1/2; π = 0.942min; 1/2) α = 15.167 min; T ½ (1/2) B = 107.069 min, AUC = 89.352 (μg · min) / ml;