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目的比较人类神经干细胞源肿瘤细胞系和人类神经干细胞系染色体G-显带的差异,探讨神经干细胞成瘤性机制。方法制备人类神经干细胞系和其来源的肿瘤细胞系(T1和T2)细胞分裂中期染色体,胰酶处理后,经吉姆萨染色,用cytoscan-karyotyping FISH ﹠CGH 软件进行G-显带分析。结果人类神经干细胞源肿瘤细胞系染色体发生缺失、易位、双着丝粒等结构改变以及染色体数目改变,核型从46,XX到69,XX不等。结论人类神经干细胞来源的肿瘤细胞系染色体发生异常改变,表现为数量变化和结构重排。
Objective To compare the difference of G-banding between human neural stem cell-derived cancer cell line and human neural stem cell line, and to explore the mechanism of neural stem cell tumorigenesis. METHODS: The metaphase chromosomes of human neural stem cell lines and their tumor cell lines (T1 and T2) were prepared. After trypsinization, the cells were stained with Giemsa and analyzed by G-banding with cytoscan-karyotyping FISH & CGH software. Results Chromosomal deletion, translocation, dicentrics and other structural changes as well as chromosome number changes were found in human NSC-derived tumor cell lines. Karyotypes varied from 46, XX to 69 and XX. Conclusion The chromosomal abnormalities in human NSC-derived tumor cell lines are characterized by quantitative changes and structural rearrangements.