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目的探讨转录活化因子3(STAT3)信号通路在纳米硒对结直肠癌细胞的作用。方法用1、3、6、12μmol/L纳米硒的细胞生长液作用于结直肠癌细胞HT29,SW620,SW480,作用6、12、24、48、72、96 h后MTT检测细胞增殖情况。流式细胞仪检测结直肠癌细胞SW480经4μmol/L纳米硒作用48 h后细胞凋亡和细胞周期。Western blot检测结直肠癌细胞SW480经4μmol/L纳米硒作用48 h后细胞中STAT3、p-STAT3、Cyclin A1的表达水平。结果纳米硒对结直肠癌细胞增殖均有抑制作用,且随作用时间和浓度增加而增加。结直肠癌细胞HT29,SW620,SW480经纳米硒作用48 h后的半数抑制浓度分别为:(6.8±0.6)、(10.4±1.1)、(4.2±0.4)μmol/L。4μmol/L纳米硒作用后的SW480细胞凋亡率明显高于0μmol/L(P<0.01)。纳米硒作用后的结直肠癌细胞中Bax表达增多,Bcl-2表达降低,G0/G1期细胞百分比与0μmol/L组相比明显降低(P<0.01),S期、G2/M期细胞百分比明显增高(P<0.01)。4μmol/L纳米硒作用后的结直肠癌细胞中p-STAT3、Cyclin A1水平与0μmol/L相比显著下降(P<0.01),而STAT3表达水平无变化。结论纳米硒可以阻断STAT3信号通路抑制结直肠癌细胞增殖,促进细胞凋亡,阻滞细胞周期。
Objective To investigate the effect of transcription factor-3 (STAT3) signaling pathway on colorectal cancer cells induced by nano-selenium. Methods Colorectal cancer cells HT29, SW620 and SW480 were treated with 1,3,6,12 μmol / L selenium. Cell proliferation was detected by MTT assay after 6, 12, 24, 48, 72 and 96 hours. Flow cytometry was used to detect the apoptosis and cell cycle of colorectal cancer SW480 cells treated with 4μmol / L nano-selenium for 48 hours. Western blot was used to detect the expression of STAT3, p-STAT3 and Cyclin A1 in colorectal cancer cells SW480 after 4μmol / L nano-selenium for 48 h. Results Nano-selenium had inhibitory effects on the proliferation of colorectal cancer cells, and increased with time and concentration. The median inhibitory concentrations of colorectal cancer cells HT29, SW620 and SW480 after 48 h of nano-selenium treatment were (6.8 ± 0.6), (10.4 ± 1.1) and (4.2 ± 0.4) μmol / L, respectively. The apoptosis rate of SW480 cells treated with 4μmol / L nano-selenium was significantly higher than that of 0μmol / L (P <0.01). The percentage of cells in G0 / G1 phase was significantly lower than that in 0μmol / L group (P <0.01), the percentage of cells in S phase and G2 / M phase was significantly higher than that in 0μmol / L group Was significantly higher (P <0.01). The expression of p-STAT3 and Cyclin A1 in 4μmol / L nano-selenium decreased significantly compared with 0μmol / L (P <0.01), while the expression of STAT3 did not change. Conclusion Nano-selenium can block the STAT3 signaling pathway to inhibit the proliferation of colorectal cancer cells, promote cell apoptosis and arrest the cell cycle.