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PCR产物检测方法的灵敏度对临床诊断结果有较大的影响。我们对端粒酶催化亚基蛋白质(hTRT)mRNA[1]的RT-PCR扩增产物琼脂糖凝胶电泳EB染色法和聚丙烯酰胺凝胶电泳银染法进行比较,以期在PCR检测方面引起重视。1材料和方法1.1材料HL-60...
The sensitivity of the PCR product detection method has a greater impact on clinical diagnosis results. We compared the RT-PCR products of telomerase catalytic subunit protein (hTRT) mRNA [1] by agarose gel electrophoresis with EB staining and polyacrylamide gel electrophoresis with silver staining in order to cause PCR detection Pay attention. 1 Materials and methods 1.1 Materials HL-60 ...