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目的应用基因芯片分析技术,筛选乳腺癌多药耐药细胞系相关基因,探讨乳腺癌化疗耐药机制。方法阿霉素持续递增作用于MCF-7细胞株,建立多药耐药细胞系MCF-7/ADM;利用Human Expr 12x135K Arr Del芯片检测MCF-7/ADM与MCF-7的差异表达基因,并利用荧光定量PCR验证差异最为显著的基因。结果不同的细胞系中筛选出差异有显著性意义的基因1520个,其中MDR1、p170、p65皆为耐药途径中较为关键的几个蛋白,p170与p65主要表现在蛋白活性的差异上,MDR1的高表达则是耐药株的显著特征。结论乳腺癌耐药涉及多种途径、多个基因,基因芯片分析技术可为乳腺癌多药耐药细胞系相关基因提供全面的技术分析。
Objective To screen the genes related to multi-drug resistant breast cancer cell lines by gene chip analysis and to explore the mechanism of drug resistance in breast cancer. METHODS: Adriamycin was continuously added to MCF-7 cell line to establish multidrug resistant cell line MCF-7 / ADM. Human Expr 12x135K Arr Del chip was used to detect differentially expressed genes in MCF-7 / ADM and MCF-7 cells. Fluorescent quantitative PCR was used to verify the most significant of the genes. Results A total of 1520 differentially expressed genes were screened out in different cell lines. Among them, MDR1, p170 and p65 were the most important proteins in the drug resistance pathway. P170 and p65 mainly expressed differences in protein activity. MDR1 The high expression of resistant strains is a significant feature. Conclusion There are many pathways involved in the drug resistance of breast cancer. Multiple gene and microarray analysis techniques can provide a comprehensive technical analysis of the genes involved in multi-drug resistant breast cancer cell lines.