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目的 研究N,N’-二乙酰-L-胱氨酸(DiNAC)对免疫性肝衰竭的治疗作用。方法 观察DiNAC对Balb/C小鼠由半乳糖胺联用脂多糖引起免疫性肝衰竭的作用。半乳糖胺/脂多糖攻击6 h后,小鼠血清ALT,AST和外周血T细胞亚群分别用全自动生化仪、流式细胞仪测定,并用光镜观察肝组织病理切片,统计半乳糖胺/脂多糖攻击24 h后的小鼠存活率。结果 给肝衰竭小鼠ip DiNAC(50,200,800 mg·kg-1),能明显阻止小鼠血清ALT和AST活力增高,使肝组织损害减轻及提高小鼠存活率,并呈剂量依赖关系;DiNAC能增强免疫性肝衰竭小鼠外周血CD4+,CD8+,Th1和Th2 T淋巴细胞的增殖分化。结论 DiNAC对免疫性肝衰竭动物有明显的治疗作用,这一作用与其免疫调节有关。
Objective To investigate the therapeutic effect of N, N’-diacetyl-L-cystine (DiNAC) on immune liver failure. Methods The effect of DiNAC on immunological liver failure induced by galactosamine and lipopolysaccharide in Balb / C mice was observed. Six hours after the challenge with galactosamine and LPS, the serum ALT, AST and T lymphocyte subsets in peripheral blood were measured by automatic biochemical analyzer and flow cytometry, respectively. The pathological sections of liver were observed with light microscope, and statistics of galactosamine / Lipopolysaccharide challenge 24 h after the mouse survival rate. The results showed that ip DiNAC (50, 200, 800 mg · kg-1) in mice with hepatic failure could obviously prevent the increase of serum ALT and AST, reduce the damage of liver tissue and increase the survival rate of mice in a dose-dependent manner; DiNAC could enhance Proliferation and Differentiation of CD4 +, CD8 +, Th1 and Th2 T Lymphocytes in Peripheral Blood of Immune Hepatic Failure Mice. Conclusion DiNAC has a significant therapeutic effect on immune liver failure animals, and its role is related to its immune regulation.