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目的探讨急性肺损伤(ALI)新生儿血清中血小板活化因子(PAF)、TNF-α的表达以及肺表面活性物质(PS)对PAF、TNF-α表达的影响。方法选择ALI患儿20例作为研究对象,分别于PS治疗前及治疗24 h、72 h,取外周静脉血2 mL,采用酶联免疫吸附试验定量测定血清PAF、TNF-α水平,同时进行血气分析,并对不同时间段血清PAF、TNF-α水平及动脉血氧分压[pa(O2)]/吸氧体积分数(FiO2)进行对比分析。结果经PS治疗24 h,PAF、TNF-α水平与治疗前比较明显下降,差异有统计学意义(P<0.05),pa(O2)/FiO2明显升高,与治疗前对比,差异有统计学意义(P<0.01);治疗72 h,PAF、TNF-α、pa(O2)/FiO2与治疗前对比,差异均有统计学意义(Pa<0.01)。结论 PS能够减少ALI新生儿PAF、TNF-α的表达,改善氧合和临床表现。
Objective To investigate the effects of pulmonary surfactant (PS) on the expression of platelet-activating factor (PAF) and tumor necrosis factor-α (PAF) and TNF-α in neonates with acute lung injury (ALI). Methods Twenty children with ALI were selected as study subjects. Serum levels of PAF and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA) before treatment, 24 h and 72 h respectively, The levels of PAF, TNF-α and arterial oxygen tension [pa (O2)] / oxygen fraction (FiO2) in different time periods were compared and analyzed. Results After treatment with PS for 24 h, the levels of PAF and TNF-α were significantly decreased compared with those before treatment (P <0.05), pa (O2) / FiO2 was significantly increased, and the difference was statistically significant (P <0.01). Compared with those before treatment, the differences of PAF, TNF-α, pa (O2) / FiO2 after 72 h of treatment were statistically significant (Pa0.01). Conclusion PS can reduce the expression of PAF and TNF-α in neonates with ALI and improve oxygenation and clinical manifestations.