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分离人血白细胞同时,以~3H一组氨酸掺入,使其在嗜碱细胞内脱羧,转化为~3H一组胺重新释放,从而得到相对的释放率.应用于变应原触发的人白细胞组胺释放模型,表现出典型剂量及动力学曲线;与临床诊断的符合率达70%以上;与荧光法测定结果密切相关(r0.71);批内RER0.0388;5ml全血可供测定3个抗原的各3个浓度(20管).除灵敏及操作简便外,由于本法不受荧光干扰物影响,在Ⅰ型变态反应研究中可能有更广泛应用.
In the meantime, human leukocytes were isolated and incorporated into ~ 3H-histidine to decarboxylate in basophils and converted into ~ 3H group of amines to be released again to obtain the relative release rate.Application to allergen-triggered humans The leukocyte histamine release model showed a typical dose and kinetic curve, and the coincidence rate with clinical diagnosis was over 70%. It was closely related to the result of fluorescence spectrometry (r0.71); intradermal RER0.0388; 5ml whole blood was available Determination of the three antigens of the three concentrations (20 tubes.) In addition to sensitivity and ease of operation, because this method is not affected by fluorescent interference, type Ⅰ allergy research may be more widely used.