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采用乙腈直接沉淀血浆蛋白 ,以头孢拉定为内标 ,建立了适合人血浆中头孢克罗分离测定的高效毛细管电泳方法。在 4 8cm× 50μm未涂层石英毛细管柱上 ,选择 50 mmol/L磷酸二氢钠 -1 2 .5mmol/L硼砂( p H8.0 8)为运行缓冲液 ,操作电压 2 0 k V( +)→ ( -) ,压力进样 1 2 psi×s,柱上紫外 2 65nm检测 ,头孢克罗和内标头孢拉定在 6.5min内获得了理想的分离 ,内源性物质不干扰高效毛细管电泳定量分析。血药浓度在 0 .2 5～ 2 5.0μg/ml范围内线性关系良好 ( r=0 .9993 ,n=5) ,绝对回收率大于 90 .5% ,日内及日间精密度均小于 9.5% ,并成功地应用于健康志愿者单剂量口服头孢克罗普通胶囊后血药浓度的动态分析。
Plasma was directly precipitated by acetonitrile, and cefradine was used as an internal standard to establish a high performance capillary electrophoresis method suitable for cefaclor separation in human plasma. On a 48 cm × 50 μm uncoated quartz capillary column, 50 mmol / L sodium dihydrogenphosphate-12.5 mmol / L borax (p H8.0 8) was used as the running buffer and the operating voltage was 20 kV (+ ) → (-), pressure injection of 1 2 psi × s, detection of UV at 65 nm on the column, cefaclor and internal standard cephradine achieved an ideal separation within 6.5 min, endogenous substances did not interfere with quantitative analysis by high performance capillary electrophoresis . The linearity was good (r = 0.9993, n = 5) in the range of 0.52 ~ 5.0μg / ml and the absolute recovery was more than 90.5%. The intra- and inter-day precision was less than 9.5% , And was successfully applied to the dynamic analysis of plasma concentrations of cefaclor capsules after oral administration of a single dose to healthy volunteers.