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目的:探讨核转录因子NF-κB/RelB基因沉默的树突状细胞(RNAiRelB dendritic cell,RNAiRelBDC)诱导异基因CD4+CD25+双阳性T细胞生成的生物效应。方法:实验分为Control-DC组、LPS-DC组、RNAiRelBDC组和LPS-RNAiRelBDC组,各组DC均与同种异基因T淋巴细胞混合反应后,采用流式细胞术(FCM)分析各组CD4+CD25+双阳性T细胞生成比例。结果:Control-DC组DC较LPS-DC组DC诱导CD4+CD25+双阳性T细胞的增高幅度更显著,两者差异具有统计学意义(P<0.01)。RNAiRelBDC组和LPS-RNAiRelBDC组DC诱导CD4+CD25+双阳性T细胞生成比例与LPS-DC组相比均存在显著差异(P<0.01),RNAiRelBDC组DC诱导CD4+CD25+双阳性T细胞生成能力较Control-DC组强,差异具有统计学意义(P<0.05)。结论:RelB基因被沉默后DC具有较强的诱导CD4+CD25+双阳性T细胞生成的能力,表现出未成熟DC的特点,这种RNAiRelBDC有望成为一种新型的致耐受DC用于免疫耐受的诱导研究。
Objective: To investigate the biological effects of RNAiRelB dendritic cell (RNAiRelBDC) induced by nuclear factor NF-κB / RelB gene on the induction of allogeneic CD4 + CD25 + double positive T cells. Methods: The experiment was divided into Control-DC group, LPS-DC group, RNAiRelBDC group and LPS-RNAiRelBDC group. After all the DCs were mixed with allogeneic T lymphocytes and analyzed by flow cytometry (FCM) CD4 + CD25 + double positive T cell generation ratio. Results: Compared with LPS-DC group, the increase of CD4 + CD25 + double positive T cells in DCs in Control-DC group was more significant (P <0.01). The percentage of CD4 + CD25 + double positive T cells induced by DC in RNAiRelBDC group and LPS-RNAiRelBDC group was significantly higher than that in LPS-DC group (P <0.01). The DCs induced by CD4 + CD25 + double positive T cells in RNAiRelBDC group were significantly -DC group, the difference was statistically significant (P <0.05). CONCLUSIONS: DCs with RelB gene silencing have a strong ability of inducing CD4 + CD25 + double positive T cells to express characteristics of immature DCs, and this RNAiRelBDC is expected to become a new type of tolerogenic DC for immune tolerance Induction studies.