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报道用Iodogen法研究了SOD的~(126)I标记。本法采用正交设计La(3~4)表对影响标记的3个因素(Iodogen 量、标记温度和标记时间),每个因素的3个水平进行了试验,仅做9组实验,就确定了最佳标记条件。标记产物~(126)I—SOD 用Sephadex G25色层柱进行分离,γ放射性测量确定标记率,纸色层法测定放化纯度,肾上腺素自氧化分光光度法测定相对活性。结果表明,对20μgSOD,Iodogen 量为50μg,标记温度为15℃,标记反应时间为15min 时,~(126)I 的标记率可达79.0%,~(126)I—SOD 的放化纯度为95.7%,相对活性为98.2%。估算~(126)I—SOD 产品比活度为2.92×10~(6)Bq/μgSOD。同时还作了提高产品比活度和~(126)I—SOD 稳定性的实验。
The Iodogen method was used to study the ~ (126) I labeling of SOD. In this method, three factors affecting the labeling (Iodogen amount, labeling temperature and labeling time), three levels of each factor were tested using the orthogonal design La (3-4) table. Only 9 sets of experiments were performed to determine The best marking conditions. The labeled product ~ (126) I-SOD was separated on a Sephadex G25 chromatographic column. The radiolabeled radioactivity was measured by radioimmunoassay. The radiochemical purity was determined by paper chromatography and the relative activity was measured by epinephrine autoxidation spectrophotometry. The results showed that the labeling efficiency of ~ (126) I was 79.0% and the radiochemical purity of ~ (126) I-SOD was 95.7 at the detection temperature of 15 ℃ and the labeling reaction time of 15 min for 20 μg SOD, 50 μg Iodogen, %, Relative activity was 98.2%. The specific activity of ~ (126) I-SOD product was estimated to be 2.92 × 10 ~ (6) Bq / μgSOD. At the same time also made to improve the specific activity and ~ (126) I-SOD stability experiments.