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目的 通过对分离到的汉坦病毒 (HV)免疫原性及病毒滴度的选育 ,选择出疫苗毒株及筛选出不破坏病毒血凝抗原的灭活剂和疫苗基质细胞 ,提高疫苗的规模化生产技术 ,生产出副反应轻 ,免疫原性和现场防病效果好的各种剂型的疫苗 ,满足人群免疫预防的需要。方法 分离到的HV株 ,应用多种方法进行免疫原性和动物保护试验选育 ;疫苗灭活剂筛选通过与甲醛灭活效果比较 ,观察对血凝素抗原的影响 ,以此作为判断指标 ;疫苗基质细胞建立主要通过观察病毒在各种细胞上的增殖滴度、基质细胞的贴壁时间及收液次数等方法 ,选择基质细胞。结果 从 45株病毒株中成功选育出Z10 (汉滩型 )和Z3 7(汉城型 )疫苗病毒株 ,两株疫苗免疫原性好、病毒滴度高 ;成功选择出 β 丙内酯作为疫苗灭活剂 ,它不破坏病毒的血凝素抗原 ,接种反应轻 ;建立的长爪沙鼠肾原代细胞作为疫苗基质 ,不仅病毒滴度高 ,而且贴壁效果好 ,可以做到多次收液的目的。结论 我们研制的疫苗无论是单价的Ⅰ型、Ⅱ型疫苗 ,还是双价 (Ⅰ型和Ⅱ型混合 )疫苗 ,接种人体后副反应轻 ,免疫效果好 ,现场防病效果也很理想 ,是目前控制肾综合征出血热流行最有效和经济的手段和措施 ,值得大力推广。
OBJECTIVE To select the vaccine strains and screen out the inactivated agents and vaccine stroma cells that do not destroy the virus hemagglutinating antigen and improve the scale of the vaccine by selecting the immunogenicity and virus titer of the isolated hantavirus (HV) The production technology to produce side effects of light, immunogenicity and disease prevention effect of various formulations of good vaccine to meet the needs of population immunoprophylaxis. HV strains isolated by the method of application of a variety of methods of immunogenicity and animal protection test selection; vaccine inactivator screening and formaldehyde inactivation effect compared to observe the impact of hemagglutinin antigen, as a judge; Vaccine stromal cells were established by observing the virus proliferation titers on a variety of cells, stromal cell adherent time and the number of fluid collection and other methods to select stromal cells. Results Z10 (Hantaan) and Z3 7 (Seoul) vaccine strains were successfully selected from 45 strains. The two vaccines have good immunogenicity and high virus titer. Β-propiolactone was successfully selected as vaccine Inactivation agent, which does not destroy the hemagglutinin antigen of the virus, the inoculation reaction is light; established Meriones unguaran primary kidney cells as a vaccine matrix, not only the virus titer is high, and the adhensive effect is good, and can be done multiple times Liquid purpose. Conclusions The vaccines we developed, both monovalent type I and type II vaccines as well as bivalent (mixed type I and type II) vaccines, have less adverse side effects and better immunological effects after inoculation, and are excellent in disease prevention effect at present The most effective and economical means and measures to control the spread of hemorrhagic fever with renal syndrome are worth promoting.