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目的 旨在探讨中药M对兔内皮细胞增殖的影响 ,试图阐明单味中草药品种M的活性成分促进血管生成的生物学机制。方法 建立兔胸主动脉内皮细胞体外培养模型 ,制备不同浓度的兔中药血清加入 96孔内皮细胞培养板 ,以MTT比色法测定内皮细胞增殖情况。结果 生脉素能够促进内皮细胞的增殖 (P <0 0 5 )。结论 中药M可能通过促进内皮细胞的增殖和分裂而达到其促进血管生成的作用
Objective To investigate the effect of Chinese herbal medicine M on the proliferation of rabbit endothelial cells in an attempt to elucidate the biological mechanism of the active ingredients of single Chinese herbal medicine M in promoting angiogenesis. Methods Rabbit thoracic aorta endothelial cells were cultured in vitro. Different concentrations of rabbit serum were prepared and added to 96-well endothelial cell culture plates. The proliferation of endothelial cells was measured by MTT assay. Results Shengmai could promote the proliferation of endothelial cells (P <0 05). Conclusion Chinese herbal medicine M may promote angiogenesis by promoting the proliferation and division of endothelial cells.