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目的观察微小RNA(miRNA)-146a转染H9c2心肌细胞高糖培养下核因子-κBp65(NF-κBp65)和Ⅰ型胶原表达。方法高糖培养H9c2细胞系,分为增强组(EC)、阴性对照组(NC)、空白对照组(BC)。采用Lipofectamine 2000Transfection Reagent分别转染miRNA-146a增敏剂(50nmol/L)、错义链(50nmol/L)、PBS,于48h后Real-time PCR测定miRNA-146a水平,Western blot测定NF-κBp65和Ⅰ型胶原水平。结果转染后,EC组H9c2细胞miRNA-146a水平高于NC组和BC组(P<0.05),NF-κBp65和Ⅰ型胶原蛋白表达量低于NC组和BC组(P<0.05)。结论 miRNA-146a参与了高糖培养H9c2心肌细胞纤维化,其机制与NF-κBp65和Ⅰ型胶原表达有关。
Objective To observe the expression of nuclear factor-κBp65 (NF-κBp65) and type Ⅰ collagen in H9c2 cardiomyocytes transfected with microRNA (miRNA) -146a. Methods H9c2 cell lines were cultured in high glucose and divided into EC group, negative control group and blank control group. The miRNA-146a level was detected by Real-time PCR at 48h after transfection with miRNA-146a sensitizer (50nmol / L) and Lipofectamine 2000 Transfection Reagent respectively. The expression of NF-κB p65 Type I collagen level. Results After transfection, the level of miRNA-146a in H9c2 cells in EC group was significantly higher than that in NC group and BC group (P <0.05). The expression of NF-κBp65 and collagen Ⅰ in EC group was lower than that in NC group and BC group (P <0.05). Conclusions miRNA-146a is involved in H9c2 cardiomyocyte fibrosis induced by high glucose, the mechanism of which is related to the expression of NF-κBp65 and type Ⅰ collagen.