目前普遍认为儿茶酚胺引起心肌蛋白质合成与心肌肥大的作用是通过激动α_1肾上腺素受体(α_1受体)来达到的。本工作的目的是确定α_1受体的两种亚型——α_(1a)与α_(1b)是否都参与上述作用。取1-3d乳鼠心脏,经胰酶消化后差速贴壁1.5h,取悬浮细胞以0.6-1.0×10~6/ml密度植入24孔培养板,在常规DMEM液中培养48h后加药,分为 It is generally accepted that the effect of catecholamine on cardiac protein synthesis and cardiac hypertrophy is achieved by agonizing the alpha 1 adrenergic receptor (alpha 1 receptor). The purpose of this work is to determine whether the two subtypes of alpha 1 receptor, α 1a and α 1b, are involved in these effects. Take 1-3d neonatal rat heart, digested with trypsin digestion adherent 1.5h, the suspension cells to 0.6-1.0 × 10 ~ 6 / ml density implantation 24-well plates, cultured in conventional DMEM solution after 48h Medicine, divided into