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目的:研究S期激酶相关蛋白2(Skp2)、p27、Galectin-3在子宫颈上皮内瘤变(CIN)中的表达及意义。方法:采用免疫组化二步法检测Skp2、p27、Galectin-3蛋白在CIN(60例)、慢性宫颈炎(20例)、正常宫颈(20例)中的表达。结果:Skp2、Galectin-3阳性细胞主要分布在上皮内瘤变的鳞状细胞及部分基底细胞中,p27阳性细胞主要分布在中表层非上皮内瘤变的鳞状细胞中。Skp2、Galectin-3在CINⅠ、Ⅱ、Ⅲ级中的阳性率明显高于正常宫颈、慢性宫颈炎组(P<0.05),其阳性率随CIN级别的增加而升高。Skp2在CINⅢ级中的阳性率明显高于CINⅠ级(P<0.05)。p27在CINⅡ、Ⅲ级中的阳性率明显低于正常宫颈、慢性宫颈炎组(P<0.05)。Skp2、Galectin-3与p27阳性表达呈负相关,其阳性表达在CIN中与患者民族、年龄无关(P>0.05)。结论:Skp2高表达和p27低表达,与宫颈恶性肿瘤的发生、发展有关。Skp2、p27联合检测有助于慢性宫颈炎与CIN的鉴别。Galectin-3在宫颈CIN中可能起促进作用。
Objective: To investigate the expression and significance of S-phase kinase-related protein 2 (Skp2), p27 and Galectin-3 in cervical intraepithelial neoplasia (CIN). Methods: The expression of Skp2, p27 and Galectin-3 protein in CIN (60 cases), chronic cervicitis (20 cases) and normal cervix (20 cases) were detected by immunohistochemical two-step method. Results: The positive cells of Skp2 and Galectin-3 mainly distributed in the squamous cells and some basal cells of intraepithelial neoplasia. The positive cells of p27 were mainly located in the non-intraepithelial neoplasia of squamous cell. The positive rates of Skp2 and Galectin-3 in CINⅠ, Ⅱ and Ⅲ were significantly higher than those in normal cervix and chronic cervicitis group (P <0.05). The positive rates of Skp2 and Galectin-3 were increased with the increase of CIN level. The positive rate of Skp2 in CINⅢlevel was significantly higher than that in CINⅠlevel (P <0.05). The positive rate of p27 in CINⅡ and Ⅲlevel was significantly lower than that in normal cervix and chronic cervicitis (P <0.05). The expression of Skp2 and Galectin-3 was negatively correlated with the expression of p27. The positive expression of Skp2 and Galectin-3 was not related to the patient’s age and ethnicity in CIN (P> 0.05). Conclusion: The high expression of Skp2 and the low expression of p27 are related to the occurrence and development of cervical malignant tumor. Skp2, p27 joint detection contribute to the identification of chronic cervicitis and CIN. Galectin-3 may play a catalytic role in cervical CIN.