论文部分内容阅读
本研究应用除草剂氟乐灵处理两叶一心幼苗生长点,进行同源四倍体萝卜种质诱导,对变异植株进行形态、细胞学鉴定和花粉母细胞染色体数目鉴定。结果表明,应用 0.2 mmol/L 和 1.0 mmol/L 氟乐灵处理,6个萝卜品种都获得同源四倍体植株,10 mmol/L 处理仅在 Nau-zhqh 得到同源四倍体;其中 0.2 mmol/L处理 Nau-dy 和 1.0 mmol/L 处理 Nau-xbch 获得四倍体最高诱导率(40%);四倍体种质与二倍体种质相比,形态性状、气孔大小、保卫细胞内叶绿体数目、花器官大小、花粉粒大小及花粉萌发率都存在显著差异,将形态、气孔鉴定和染色体计数结合可以准确确定变异株的倍性。研究表明利用氟乐灵诱导是进行萝卜同源四倍体种质创新的有效途径之一。本研究应用除草剂氟乐灵处理两叶一心幼苗生长点,进行同源四倍体萝卜种质诱导,对变异植株进行形态、细胞学鉴定和花粉母细胞染色体数目鉴定。结果表明,应用 0.2 mmol/L 和 1.0 mmol/L 氟乐灵处理,6个萝卜品种都获得同源四倍体植株,10 mmol/L 处理仅在 Nau-zhqh 得到同源四倍体;其中 0.2 mmol/L处理 Nau-dy 和 1.0 mmol/L 处理 Nau-xbch 获得四倍体最高诱导率(40%);四倍体种质与二倍体种质相比,形态性状、气孔大小、保卫细胞内叶绿体数目、花器官大小、花粉粒大小及花粉萌发率都存在显著差异,将形态、气孔鉴定和染色体计数结合可以准确确定变异株的倍性。研究表明利用氟乐灵诱导是进行萝卜同源四倍体种质创新的有效途径之一。
In this study, the herbicide trifluralin was used to treat the growth of two leaves and one heart of seedling. The autotetraploid radish germplasm was induced. The morphological and cytological identification of mutant plants and the chromosome number identification of pollen mother cells were carried out. The results showed that autotetraploid plantlets were obtained from 6 radish cultivars treated with 0.2 mmol / L and 1.0 mmol / L trifluralin, and autotetraploid plants were obtained from 10 mmol / L Nau-zhqh only. 0.2 Compared with diploid germplasm, the tetraploid germplasm had the highest morphological traits, the size of stomata, the number of guard cells, and the highest induction rate of tetraploid (40%) in the treatment of Nau-dy and 1.0 mmol / L Nau-xbch. There were significant differences in the number of internal chloroplasts, flower organs, pollen grains and pollen germination rate. Studies have shown that the use of trifluralin induction is one of the effective ways to radish autotetraploid germplasm innovation. In this study, the herbicide trifluralin was used to treat the growth of two leaves and one heart of seedling. The autotetraploid radish germplasm was induced. The morphological and cytological identification of mutant plants and the chromosome number identification of pollen mother cells were carried out. The results showed that autotetraploid plantlets were obtained from 6 radish cultivars treated with 0.2 mmol / L and 1.0 mmol / L trifluralin, and autotetraploid plants were obtained from 10 mmol / L Nau-zhqh only. 0.2 Compared with diploid germplasm, the tetraploid germplasm had the highest morphological traits, the size of stomata, the number of guard cells, and the highest induction rate of tetraploid (40%) in the treatment of Nau-dy and 1.0 mmol / L Nau-xbch. There were significant differences in the number of internal chloroplasts, flower organs, pollen grains and pollen germination rate. Studies have shown that the use of trifluralin induction is one of the effective ways to radish autotetraploid germplasm innovation.