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目的-建立一种清醒小鼠严重颅脑闭合性撞击伤模型, 模拟交通事故和战伤中的清醒致伤过程, 为相关研究创造新的动物模型基础。方法-将雄性KM小鼠头戴钢盔后清醒固定, 用BIM III型小型多功能动物撞击机击打, 致伤后2 h、8 h、24 h、48 h、72 h、120 h、1 w、2 w进行神经行为学评分、运动功能评分、死亡小鼠脑组织和肺组织水含量、死亡率、病理切片和电镜观察。结果-致伤后 48 h内致伤组运动功能评分、神经行为学评分明显低于对照组 (P<0 05); 致伤后死亡率高, 各组死亡小鼠脑组织水含量无明显差异, 但致伤组肺组织水含量明显高于对照组 (P <0 05); 病理切片和电镜检查显示, 致伤后脑组织细胞肿胀、坏死。结论-基本成功建立了模拟交通事故和战伤的清醒小鼠严重颅脑闭合性撞击伤模型。
OBJECTIVE: To establish a model of severe traumatic brain injury in conscious mice and to simulate the process of wakeful injury in traffic accidents and war injuries and to create a new animal model foundation for related research. Methods - Male KM mice were awakened after wearing a helmet and were beaten with a small multi-functional animal impact machine (BIM III). After 2 h, 8 h, 24 h, 48 h, 72 h, 120 h, 1 w , 2 w neurobehavioral score, motor function score, dead mice brain tissue and lung tissue water content, mortality, pathological sections and electron microscopy. Results - Within 48 hours after injury, motor function score and neurobehavioral score of the injured group were significantly lower than those of the control group (P <0.05); the mortality rate after injury was high, and there was no significant difference in the brain water content of the dead mice in each group , But the water content in the lung tissue of the injured group was significantly higher than that of the control group (P <0.05). Pathological examination and electron microscopy showed that the brain tissue was swollen and necrotic after the injury. Conclusion - The successful establishment of a model of severely craniocerebral injury caused by traffic accident and war-wounded mice was basically successful.