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目的探讨科罗索酸对人结肠癌Lo Vo细胞株裸鼠移植瘤生长的影响极其作用机制。方法 Lo Vo细胞接种于裸鼠皮下制备裸鼠移植瘤模型。将造模成功的小鼠随机分成4组,分别灌胃给予0 mg/kg、5 mg/kg、10 mg/kg、20 mg/kg科罗索酸。每3 d测量肿瘤体积,连续给药24 d后剥离瘤块称重,计算抑瘤率;TUNEL技术检测细胞凋亡情况,Western blot检测细胞总STAT3及p-STAT3的表达。结果科罗索酸低、中、高剂量组的抑瘤率分别是18.1%,45.7%(P<0.05)和63.6%(P<0.01),肿瘤细胞的凋亡比率分别是(7.7±2.8)%,(13.3±4.3)%(P<0.05),(18.9±5.1)%(P<0.01),并呈剂量依赖性的抑制STAT3活化。结论科罗索酸可抑制Lo Vo裸鼠移植瘤的生长,促进细胞凋亡,其机制可能与抑制STAT3的活化有关。
Objective To investigate the effect of corosolic acid on the growth of human colon cancer LoVo cell xenografts in nude mice. Methods LoVo cells were inoculated subcutaneously in nude mice to establish a nude mouse xenograft model. The successful mice were randomly divided into 4 groups and fed with 0 mg / kg, 5 mg / kg, 10 mg / kg and 20 mg / kg corosolic acid respectively. The volume of tumor was measured every 3 days, and the tumor mass was weighed 24 days after continuous administration. The tumor inhibition rate was calculated. The apoptosis of cells was detected by TUNEL technique. The expression of STAT3 and p-STAT3 were detected by Western blot. Results The antitumor rates of corosolic acid in low, medium and high dose groups were 18.1%, 45.7% (P <0.05) and 63.6% (P <0.01), respectively. The rates of apoptosis in tumor cells were (7.7 ± 2.8) %, (13.3 ± 4.3)% (P <0.05), (18.9 ± 5.1)% respectively (P <0.01). Conclusion Corosolic acid can inhibit the growth of xenografts in nude mice and promote apoptosis. The mechanism may be related to the inhibition of STAT3 activation.