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以美味猕猴桃雄株茎段愈伤组织为材料，分离原生质体，并培养在ＫＭ８Ｐ附加０．４５ｍｏｌ／Ｌ葡萄糖和０．０５ｍｏｌ／Ｌ蔗糖的培养基上，用低熔点琼脂糖包埋，６～７ｄ发生第一次细胞分裂，培养２０ｄ的分裂频率为１１．３％．在未添加新鲜培养液的情况下，原生质体再生的细胞可持续分裂至８０ｄ左右，并形成２～３ｍｍ大小的愈伤组织．然后采用二步诱导分化法将原生质体来源的愈伤组织诱导分化出绿苗，再诱导生根，形成完整的小植株．
The protoplasts were isolated from the stem segments of male offspring of Actinidia deliciosa and cultured on KM8P supplemented with 0.45 mol / L glucose and 0.05 mol / L sucrose, embedded in low melting point agarose, The first cell division took place on day 7 and the division frequency on day 20 was 11.3%. In the absence of fresh culture medium, the protoplast regeneration of cells can continue to divide to 80d or so, and the formation of 2 ~ 3mm size callus. Then, two-step induction and differentiation method was used to induce the protoplast-derived callus to differentiate into green seedlings and induce rooting to form a complete plantlet.