本文报道,一例由流产布鲁氏菌引起的慢性骨髓炎,仅用缓冲炭末酵母浸液(BCYE)培养基分离出布鲁氏菌才得以确诊。患者35岁,男,左大腿进行性疼痛4年就医。x 线透视表明,左股骨远端髓腔有一扩张性溶骨病损区。活检病理报告为坏死性肉芽肿,抗酸杆菌和真菌染色及培养均阴性。PPD 试验;组织胞浆菌病、隐球菌病,芽生菌病与孢子丝菌病等的血清学反应; Here we report that one case of chronic osteomyelitis caused by Brucella abortus was diagnosed only by Brucella fermentation with buffer charcoal yeast extract (BCYE). Patient 35 years old, male, left thoracic pain 4 years to seek medical treatment. X-ray showed that the left femoral distal medullary cavity has an expansion of osteolytic lesions. Biopsy pathology report of necrotizing granuloma, acid-fast bacilli and fungus staining and culture were negative. PPD test; histoplasmosis, cryptococcosis, blastomycosis and sporotrichosis and other serological reactions;