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以没食子酸为标样,研究Folin-Ciocalteu比色法测定枳椇果梗总酚含量的适宜条件。结果表明,枳椇果梗提取物加Folin-Ciocalteu试剂0.6mL、20g/100mL Na2CO3溶液1.5mL,定容至10mL,30℃避光反应60min,测定反应体系在750nm处的吸光度,多酚质量浓度在0～8.47mg/L范围内与吸光度有良好的线性关系。该方法的稳定性、重现性、精密度以及加标回收率的相对标准偏差均符合要求,可用于枳椇果梗多酚含量的测定。
With gallic acid as the standard sample, Folin-Ciocalteu colorimetric determination of the optimum conditions for the total phenolics of dulcis dulcis peduncle. The results showed that 0.6mL Folin-Ciocalteu reagent, 1.5mL 20g / 100mL Na2CO3 solution, 10mL volumetric flask and 60 ℃ dark reaction at 30 ℃, the absorbance of the reaction system at 750nm, the concentration of polyphenols In the range of 0 ~ 8.47mg / L there is a good linear relationship with absorbance. The stability, reproducibility, precision and standard deviation of spike recoveries of the method meet the requirements and can be used to determine the content of polyphenol in Hovenia dulcis Thunb.