论文部分内容阅读
目的:探讨肉苁蓉苯乙醇苷对高原肺动脉高压大鼠肺血流动力学的影响。方法:Wistar大鼠72只,随机分为正常对照组12只及造模组60只,正常对照组常压常氧SPF环境饲养;造模组模拟海拔5 000 m高原环境人工实验舱中饲养4周制备高原肺动脉高压模型。再随机将造模组分为模型组,大花红景天组(1.78 mL·kg-1·d-1),肉苁蓉苯乙醇苷低中高剂量组(75,150,300 mg·kg-1·d-1),每组12只。第5周开始正常对照组、模型组灌胃给予生理盐水,各治疗组按计量灌胃给药28 d,每日1次,给药体积10 mL·kg-1。主动脉插管法测定平均动脉压(MAP),直接法测定平均肺动脉压(mPAP),右心室收缩压(RVSP),计算右心肥厚指数(RVHI),观察各组大鼠肺组织病理改变并计算中膜厚度比(WT)和中膜面积比(WB)。结果:模型组肺动脉血管增厚度,炎性浸润,WT,WB,mPAP,RVSP,RVHI较正常对照组显著增加(P<0.01);大花红景天及肉苁蓉苯乙醇苷低中高剂量组与模型组相比均能改善肺动脉血管增厚、炎性浸润,降低WT及WB,mPAP,RVSP,MAP,RVHI(P<0.01),但仍高于正常对照组(P<0.01)。结论:肉苁蓉苯乙醇苷可以改善高原肺动脉高压大鼠的肺血流动力学、右心室肥厚程度及肺组织病理改变,具有一定治疗高原肺动脉高压的作用。
Objective: To investigate the effects of Cistanche Pontiff on pulmonary hemodynamics in rats with plateau pulmonary hypertension. Methods: Seventy-two Wistar rats were randomly divided into normal control group (n = 12) and model group (n = 60). The normal control group was fed with normoxia and aerobic SPF environment. The model group was reared at altitude of 5 000 m Week preparation of high altitude pulmonary hypertension model. The model groups were randomly divided into model group, Rhodiola rosea group (1.78 mL · kg-1 · d-1), Cistanche etongalide group (75,150,300 mg · kg-1 · d-1) 12 in each group. At the fifth week, the normal control group was started. The model group was given gavage with normal saline. Each treatment group was given gavage for 28 days, once a day. The volume of administration was 10 mL · kg-1. The aortic cannulation method was used to measure mean arterial pressure (MAP). The mean pulmonary arterial pressure (mPAP) and right ventricular systolic pressure (RVSP) were measured by direct method. The right ventricular hypertrophy index (RVHI) Media thickness ratio (WT) and media area ratio (WB) were calculated. Results: Compared with normal control group, the thickening of pulmonary artery, inflammatory infiltration, WT, WB, mPAP, RVSP and RVHI in model group were significantly increased (P <0.01) (P <0.01), but still higher than that of the normal control group (P <0.01). CONCLUSION: Cistancin acetophenoside can improve pulmonary hemodynamics, right ventricular hypertrophy and lung histopathology in rats with plateau pulmonary hypertension, and has the effect of treating altitude pulmonary hypertension.