摘要 表达序列标签(EST)策略是寄生虫基因组研究的工作重点之一,它在血吸虫研究中的成功应用可大大增加对血吸虫基因和血吸虫本质的认识,并为确定新的血吸虫候选疫苗和药物靶标提供更多的候选基因,揭开了血吸虫基因组研究的新篇章。本文就EST的基本概念、EST策略及其在血吸虫基因发现、基因表达谱分析中的应用作一简要综述。 Abstract The expressed sequence tag (EST) strategy is one of the most important work in the field of parasite genomes. Its successful application in schistosomiasis research will greatly increase the understanding of the nature of schistosoma gene and schistosomiasis. In order to identify new Schistosoma vaccine candidates and drug targets Provide more candidate genes, opened a new chapter in schistosome genome research. This article briefly reviews the basic concept of EST, EST strategy and its application in gene discovery and gene expression profiling of schistosomiasis.