论文部分内容阅读
目的探讨大鼠空间辨别性学习记忆活动与趋化因子基质细胞衍生因子-1(SDF-1)的关系。方法用免疫组织化学方法、免疫印迹法和RT-PCR方法对获得空间辨别性学习记忆大鼠海马结构内SDF-1表达进行研究,并与游水组和对照组比较。结果1.免疫组织化学方法显示,对照组大鼠海马结构内趋化因子SDF-1阳性细胞分布在海马结构各亚区和各层。阳性细胞的形态以圆形为主,少见锥体形和梭形。阳性产物主要分布在细胞膜上;游水组大鼠海马结构内SDF-1阳性细胞的形态和分布与对照组相比未见组间差异;训练组大鼠海马结构内SDF-1免疫阳性细胞的数量比对照组增多(P<0.01)。阳性细胞的形态以锥体形和三角形居多,在分布上有向齿状回聚集的趋势。2.免疫印迹方法检测训练组大鼠海马结构内SDF-1蛋白质的表达明显高于对照组(P<0.01),而且在训练组内则随着训练时间的延长表达量随之增加。3.RT-PCR方法检测训练组大鼠海马结构内SDF-1mRNA的量明显高于对照组(P<0.01),且随着训练时间的延长表达增加。在水盆中漫游的游水组大鼠,不论是免疫组织化学方法,还是免疫印迹法以及RT-PCR方法获得的结果与对照组相比均未见组间差异。结论经水迷宫训练使大鼠获得空间辨别性学习记忆功能,致使海马结构内细胞导向因子SDF-1表达上调,上调的SDF-1蛋白质可能与所获功能需要的形态基础-神经微环路的形成有关。
Objective To investigate the relationship between spatial discrimination learning and memory activities and chemokine stromal cell derived factor-1 (SDF-1) in rats. Methods The expression of SDF-1 in hippocampus of spatial learning and memory rats was studied by immunohistochemistry, immunoblotting and RT-PCR. The expression of SDF-1 in hippocampal formation was compared with that in swimming and control groups. Immunohistochemistry showed that SDF-1 positive cells in the hippocampal formation of hippocampal formation were distributed in all sub-regions and layers of hippocampal formation. Positive cells in the shape of the main round, rare pyramidal and fusiform. The positive products were mainly distributed on the cell membrane. The morphology and distribution of SDF-1 positive cells in hippocampal formation in swimming group were not different from those in control group. The number of SDF-1 positive cells in hippocampal formation in training group Than the control group increased (P <0.01). Positive cells in the shape of pyramids and triangles, in the distribution of the tendency to dentate aggregation trend. The expression of SDF-1 protein in hippocampal formation of rats in training group was significantly higher than that in control group (P <0.01) by immunoblotting, and the expression of SDF-1 protein increased with training time in training group. The amount of SDF-1mRNA in hippocampal formation of rats in training group was significantly higher than that of control group (P <0.01) by RT-PCR method, and increased with training time. There was no difference between the two groups in swimming group swimming in water basin, whether immunohistochemistry, immunoblotting or RT-PCR. Conclusions The water-maze training results in spatial differentiation of learning and memory in rats, resulting in the up-regulation of SDF-1 expression in the hippocampal formation. The up-regulated SDF-1 protein may be related to the morphological basis of the acquired function - Formed about.