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【目的】探讨杀螨植物牛蒡Arctium lappaL.提取物中主要杀螨成分L1-2的杀螨作用机理。【方法】采用叶片浸渍法处理桔全爪螨Panonychus citri雌成螨后,测定了静止期、兴奋期、痉挛期、麻痹期、复苏和死亡期5个中毒阶段试虫体内几种代谢酶的活性。【结果】L1-2组分在静止期和复苏期对羧酸酯酶(carboxylesterase,CarE)具有一定的抑制作用,在其他时期均激活CarE活性。除了静止期外,在其他时期均能激活乙酰胆碱酯酶(acetylcholinesterase,AChE)和谷胱甘肽转移酶(glutathione S-transferases,GSTs)的活性,在痉挛期和麻痹期活性增强,随后在麻痹期和复苏期降低。【结论】L1-2组分对CarE的抑制与其毒杀活性有关,而中毒试虫的复苏可能与AChE和GSTs有关。该组分可在较长时期内影响桔全爪螨的神经传导及消化和生殖系统,具有潜在的应用研究价值。
【Objective】 To investigate the mechanism of acaricidal action of the main acaricidal component L1-2 in Arctium lappa L. extract of acaricidal plant Arctium lappa L. 【Method】 The activity of several metabolic enzymes in the testis of Panonychus citri female adults at stationary phase, excitement phase, spastic phase, paralysis phase, resuscitation and death stages were measured by leaf dipping method. . 【Result】 L1-2 had some inhibitory effects on carboxylesterase (CarE) in stationary phase and in resuscitation phase, and activated CarE activity in other phases. In addition to quiescence, AChE and glutathione S-transferases (GSTs) were activated at other times, with increased activity during spasticity and paralysis, followed by paralysis And the recovery period is lower. 【Conclusion】 The inhibitory effect of L1-2 on CarE is related to its killing activity, while the recovery of poisoning worms may be related to AChE and GSTs. This component may affect the nerve conduction, digestion and reproductive system of Panonychus citri for a long time and has potential application value.