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目的了解梅州市家禽鸟类市场环境样品禽流感病毒的感染状况,及时发现人禽流感发病潜在危险因素,防止疫情发生。方法 2013年4月22日至2013年5月31日,在梅州市选取1个三鸟批发市场和5个居民区肉菜市场,以擦拭涂抹法采集外环境活禽粪便以及各种用具表面拭子,采用荧光定量PCR方法检测甲型流感病毒核酸,并进行病毒基因同源性分析;对外环境H7N9核酸阳性的市场及周边区域开展监测工作。结果 6个活禽市场共检测样品226份,检出甲型流感病毒核酸阳性24份,阳性率10.62%,其中H7N9型2份,阳性率0.88%;基因同源性分析,梅州市外环境H7N9病毒基因与广东省惠州市病例获得的H7N9禽流感病毒同源性为99.6%,与华东地区病人感染的H7N9禽流感病毒同源性为99.7%~99.8%。对外环境检出H7N9阳性市场的165名密切接触者进行医学观察,未发现发热、咳嗽等异常临床表现。结论梅州市活禽市场外环境存在甲型流感病毒污染,且病毒未发现变异,2个市场外环境H7N9禽流感病毒核酸阳性未造成人群感染。
Objective To understand the infection status of bird flu in environmental samples of poultry and birds market in Meizhou City and find out the potential risk factors of human bird flu and prevent the occurrence of the outbreak. Methods From April 22, 2013 to May 31, 2013, a three-bird wholesale market and five residential vegetable markets were selected in Meizhou, and wiped smear method was used to collect external environment live poultry excrement and various utensils surface wipe Subunit, the fluorescent quantitative PCR method was used to detect the influenza A virus nucleic acid, and the homology analysis of the virus gene was carried out; the market of H7N9 positive nucleic acid in the external environment and the surrounding area were monitored. Results A total of 226 samples were tested in 6 live poultry markets. The positive rate of influenza A virus was 24, the positive rate was 10.62%, of which 2 were H7N9 type and the positive rate was 0.88%. The homology analysis of H7N9 The homology of the virus gene to H7N9 bird flu virus obtained from Huizhou City of Guangdong Province was 99.6%, which was 99.7% -99.8% homologous to the H7N9 bird flu virus of East China patients. The external environment H7N9 positive market detected 165 close contacts of medical observation, found no fever, cough and other abnormal clinical manifestations. CONCLUSION: Influenza A virus is present in the live environment of live poultry in Meizhou City. No mutation has been found in the virus outside the market. Two positive samples of H7N9 bird flu virus from the external market did not cause any population infection.