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目的研究脑梗死后外周血淋巴细胞神经营养因子(NGF、BDNF、NT-3)及受体mRNA和相关蛋白质表达变化。方法用RT-PCR和Westernblot技术观测外周血淋巴细胞神经营养因子及受体mRNA和相关蛋白质的动态变化。结果急性期外周血淋巴细胞NT-3及受体mRNA和相关蛋白质表达较对照组明显减少(P<0·01),恢复期逐渐上升;急性期NGF与BDNF及其受体mRNA和相关蛋白质表达较对照组显著增加(P<0·01),恢复期无明显表达。结论脑梗死急性期时NGF与BDNF及其受体mRNA和相关蛋白质表达增加,而NT-3及受体mRNA和相关蛋白质表达在缺血初期下降,提示缺血早期NT-3可增加神经元死亡。
Objective To study the changes of peripheral blood lymphocyte neurotrophic factor (NGF, BDNF, NT-3) and its receptor mRNA and related proteins after cerebral infarction. Methods RT-PCR and Western blotting were used to observe the dynamic changes of peripheral blood lymphocyte neurotrophic factor and its receptor mRNA and related proteins. Results The mRNA and protein expressions of NT-3 and its receptors in peripheral blood lymphocytes were significantly decreased (P <0.01) in the acute phase and gradually recovered in the recovery phase. The expressions of NGF, BDNF and its receptor mRNA and related proteins Compared with the control group increased significantly (P <0.01), no significant recovery during convalescence. Conclusions The expression of NGF, BDNF and its receptor mRNA and related proteins increased in the acute stage of cerebral infarction, while the expression of NT-3 and its receptor mRNA and related proteins decreased in the early phase of ischemia, suggesting that NT-3 may increase the neuronal death .