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Antagonistic bacterium HJX1 is a Bacillus subtilis strain isolated from field rhizosphere soil where banana wilt was serious,with certain control efficacy on banana Fusarium wilt.The crude extract obtained from its fermentation broth after ammonium sulfate precipitation has an inhibitory effect on various pathogenic fungi.The culture conditions for this strain were optimized in this study with Fusarium oxysporum f.sp.cubense as an indicator,and the results showed that the fermentation broth obtained from culture in LB liquid medium under 30 ℃ at 170 r / min for 48 h had the strongest inhibition against F.oxysporum f.sp.cubense.It was observed that the inhibition was mainly reflected by causing breakage and distortion of mycelia,followed by appearance of vesicles and swelling,ablation,and leakage of protoplasm.This study provides a scientific basis for the study on the action mechanism of crude protein from bacterial strain HJX1.
Antagonistic bacterium HJX1 is a Bacillus subtilis strain isolated from field rhizosphere soil where banana wilt was serious, with certain control efficacy on banana Fusarium wilt.The crude extract obtained from its fermentation broth after ammonium sulfate precipitation has an inhibitory effect on various pathogenic fungi. culture conditions for this strain were optimized in this study with Fusarium oxysporum f.sp.cubense as an indicator, and the results showed that the fermentation broth obtained from culture in LB liquid medium under 30 ° C at 170 r / min for 48 h had the the strongest inhibition against F. oxysporum f.sp.cubense. It was observed that the inhibition was mainly reflected by causing breakage and distortion of mycelia, followed by appearance of vesicles and swelling, ablation, and leakage of protoplasm. this study provides a scientific basis for the study on the action mechanism of crude protein from bacterial strain HJX1.