目的 :探讨一氧化氮 (NO)与人精液转铁蛋白 (Tf)含量及精子质量的关系 .方法 :参照WHO标准 ,进行精液常规分析 .无菌取大鼠睾丸 ,经胶原酶和透明质酸酶消化 ,分离出纯度较高的支持细胞 ,用Ham sF 1 2培养液培养 .实验组加入NO供体硝普钠 (SNP) ,同对照组一起培养 4 8h取上清液 ,采用镀铜镉还原荧光法检测NO代谢产物硝酸盐 (NO-3 ) .用免疫散射比浊法检测Tf含量 .结果 :1 30例不育者精液Tf含量、精子密度和活动率随NO含量升高而降低 ,呈显著的负相关 (P <0 .0 1 ) ,大鼠睾丸支持细胞悬液Tf含量正常生育组[(2 2± 9) μmol/L]显著高于不育组 [(1 3± 8) μmol/L ,P <0 .0 1 ].结论 :精液Tf含量测定有助于评价精子质量 ,可作为反映支持细胞功能的特异指标 ,NO对精子运动能力及支持细胞产生和分泌Tf有抑制作用 ,这对研究不育症的机制有重要价值 . Objective: To investigate the relationship between nitric oxide (NO) and human semen transferrin (Tf) content and sperm quality.Methods: According to the WHO standard, routine semen analysis was performed.Septic rats were injected with collagenase and hyaluronic acid Enzyme digestion, separation of high purity of the support cells, with Ham sF 1 2 broth culture.Experimental group with NO donor nitroprusside (SNP), with the control group cultured 48 h supernatant, using copper-cadmium The nitric oxide (NO-3), a metabolite of NO, was measured by reduction fluorometry. The Tf content was detected by immune nephelometry.RESULTS: The Tf content, sperm density and activity of semen in 1 300 infertile women decreased with the increase of NO content, (P <0.01). The Tf content in testis supportive cell suspension was significantly higher in normal fertility group ([(22 ± 9) μmol / L) than that in infertility group [(13 ± 8) μmol / L, P <0.01] .Conclusion: The determination of Tf content in semen is helpful to evaluate the quality of sperm, which can be used as a specific index to reflect the function of supporting cells. NO can inhibit the sperm motility and Tf production and secretion , Which is of great value for studying the mechanism of infertility.