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目的:探讨穿心莲对糖尿病血管病变的保护机制。方法:制备糖尿病大鼠模型,并给予穿心莲干预,8周后,取血测定一氧化氮(NO)及内皮素-1(ET-1)。取胸主动环观察不同累积浓度的乙酰胆碱(Ach)对去甲肾上腺素(NE)引起的血管收缩的抑制率。另留取一段胸主动脉制备病理切片,SP法免疫组化染色,观察细胞间黏附分子-1(ICAM-1)及血管细胞间黏附分子-1(VCAM-1)的阳性表达。结果:穿心莲组与模型组相比能明显抑制升高的血浆ET-1(P<0.05);升高NO的水平(P<0.05);但均达不到正常组的水平(P<0.05)。模型组血管环对NE的收缩反应明显强于正常对照组及穿心莲组(P<0.05),且穿心莲组对NE的收缩反应亦强于正常对照组(P<0.05)。在相同Ach浓度下,模型组及穿心莲组胸主动脉环对Ach的舒张作用明显弱于正常组(P<0.05),而穿心莲组强于模型组(P<0.05)。穿心莲组能明显抑制糖尿病大鼠胸主动脉ICAM-1及VCAM-1表达(P<0.05)。结论:穿心莲具有降低大鼠ET-1及升高NO的作用,能够保护糖尿病大鼠内皮依赖的血管舒张功能,且能够抑制ICAM-1及VCAM-1的表达,具有抗动脉硬化作用。
Objective: To investigate the protective mechanism of Andrographis paniculata on diabetic vascular lesions. METHODS: Diabetic rat models were prepared and were administered with andrographolide. Eight weeks later, blood samples were taken to determine nitric oxide (NO) and endothelin-1 (ET-1). The active chest of the chest was taken to observe the inhibition rate of norepinephrine (NE)-induced vasoconstriction by different cumulative concentrations of acetylcholine (Ach). A section of thoracic aorta was also taken for preparation of pathological sections. SP immunohistochemical staining was performed to observe the positive expression of intercellular adhesion molecule-1 (ICAM-1) and vascular intercellular adhesion molecule-1 (VCAM-1). Results: Compared with the model group, compared with the model group, the andrographolide significantly inhibited the elevated plasma ET-1 (P<0.05); increased the level of NO (P<0.05); but could not reach the level of the normal group (P<0.05). . The contraction response of vascular rings to NE was significantly stronger in the model group than in the normal control group and the Andrographolide group (P<0.05), and the contraction response of the NE group was also stronger than that of the normal control group (P<0.05). At the same Ach concentration, the relaxation effect of Ach on the thoracic aorta ring in the model group and the Andrographis paniculata group was significantly weaker than that in the normal group (P<0.05), while the Andrographolide group was stronger than the model group (P<0.05). The Andrographis group significantly inhibited the expression of ICAM-1 and VCAM-1 in the thoracic aorta of diabetic rats (P<0.05). Conclusion: Andrographis paniculata has the effects of reducing ET-1 and increasing NO in rats, and can protect the endothelium-dependent vasodilatation of diabetic rats, and it can inhibit the expression of ICAM-1 and VCAM-1, and has anti-atherosclerosis effect.