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按ＷＨＯ精液参数标准，分别选择精子活力低下的男性不育症患者和正常生育力男子精液标本各２０例，将洗涤后的精子用含１％ＴｒｉｔｏｎＸ－１００的０．１ＭＴｒｉｓ－ＨＣｌ缓冲液处理，提取精子膜结合尿激酶型纤溶酶原激活因子（ｕＰＡ），然后采用抗人尿激酶多克隆抗体，进行各样品ｕＰＡ含量的测定（夹心法ＥＬＩＳＡ）。结果显示：弱精症男子精子膜结合ｕＰＡ（２３．１０±７．３５ｍｕ／１０６ｃｅｌｓ）显著性低于正常人精子膜ｕＰＡ（２９．８９±９．４８ｍｕ／１０６ｃｅｌｓ），Ｐ＜０．０２５。且精子膜ｕＰＡ含量与精子活率呈直线正相关（ｒ＝０．６４，Ｐ＜０．０００１），与精子活力（直线前向运动精子百分率）亦呈线性相关（ｒ＝０．４８，Ｐ＜０．００５）。说明精子膜结合ｕＰＡ可能与精子活力及生育力之间有一定关联。
According to the WHO semen parameter standard, 20 male infertility patients and 20 normal fertility men semen samples were selected respectively. The washed sperm were treated with 0.1M Tris-HCl buffer containing 1% TritonX-100, Extraction of sperm membrane combined with urokinase-type plasminogen activator (uPA), and then using anti-human urokinase polyclonal antibody determination of uPA content of each sample (sandwich ELISA). The results showed that the sperm membrane binding uPA (23.10 ± 7.35mu / 106cels) in asthenospermia men was significantly lower than that in normal human uPA (29.89 ± 9.48mu / 106cels), P <0.025. There was a linear correlation between sperm motility and sperm motility (r = 0.64, P <0.0001) and sperm motility (sperm motility) (r = 0.48, P <0.005). Sperm membrane binding uPA may be related to sperm motility and fertility have some relevance.