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目的检测Actin-γ在冠心病血小板中蛋白含量和外周血白细胞mRNA表达,探讨其在冠心病发病过程中的作用。方法纳入冠心病患者40例,健康对照组20例,运用流式细胞术检测单核细胞-血小板聚集体(MPAs)以观察血小板活化状态;应用蛋白免疫印迹(Western--blot)法检测血小板Actin-γ蛋白含量;采用实时荧光定量逆转录聚合酶链式反应(RT-PCR)方法检测外周血白细胞Actin-γmRNA表达。结果与健康对照组相比,冠心病组MPA水平(P<0.01)和血小板骨架蛋白Actin-γ含量明显升高(P<0.01),且二者因冠心病不同类型有所差异;而白细胞Actin-γmRNA表达无统计学差异(P>0.05)。结论冠心病发病过程中血小板骨架蛋白Actin-γ重组为血小板活化和变形、聚集提供了机械来源,可能在冠心病疾病过程中发挥了一定的作用。
Objective To detect the protein content of platelet and the mRNA expression of leukocyte in peripheral blood in patients with coronary heart disease (CHD) and to explore the role of Actin-γ in the pathogenesis of coronary heart disease. Methods Forty patients with coronary heart disease and 20 healthy controls were enrolled in this study. Monocyte - platelet aggregates (MPAs) were detected by flow cytometry to observe the platelet activation status. Western blot analysis was used to detect platelet Actin -γ protein in peripheral blood leukocytes; Real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of Actin-γmRNA in peripheral leukocytes. Results The levels of MPA and Actin-γ in coronary heart disease group were significantly higher than those in healthy control group (P <0.01), and both of them were different in different types of coronary heart disease. Actin There was no significant difference in the expression of -γ mRNA (P> 0.05). Conclusion Platelet-derived protein Actin-γ recombination is a kind of mechanical source of platelet activation, deformation and aggregation during the pathogenesis of coronary heart disease. It may play a role in coronary heart disease.